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Antibodies bound to the blocking peptide now not bind on the epitope within the concentrate on protein. By comparing the staining from the blocked antibody as opposed to the antibody on your own, you can see which staining is certain.

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This information outlines further achievable optimization of mobile and immunoassay parameters to make certain the very best results are...

This information outlines further doable optimization of mobile and immunoassay parameters to make certain the absolute best success are...

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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both antibodies...

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Blocking peptides are peptides that bind especially for the goal antibody and block antibody binding. These peptide generally consists of the epitope recognized by the antibody.

Concentrate on Information and facts Cyclin E belongs to your hugely conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which lead for the temporal coordination of each mitotic party. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress through S period.

Overexpression of this gene has long been observed in several tumors, which ends up in chromosome instability, and so may possibly contribute to tumorigenesis. This protein was observed to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a crucial purpose in marketing mobile-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct here isoforms, are explained. Two further splice variants ended up reported but thorough nucleotide sequence information just isn't still accessible.

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